Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus

G3: Genes, Genomes, Genetics

article

Maren Wellenreuther¹  Jérémy Le Luyer³  Denham Cook¹  Peter A. Ritchie⁵  Louis Bernatchez³ 

[1]The New Zealand Institute for Plant and Food Research Limited, Nelson, New Zealand

[2]School of Biological Sciences, The University of Auckland, New Zealand

[3]Institut de Biologie Intégrative et des Systèmes (IBIS), Pavillon Charles-Eugène Marchand, Université Laval, Québec, Canada

[4]Ifremer, UMR 241 Ecosystèmes Insulaires Océaniens, Centre Ifremer du Pacifique, BP 49, 98725 Tahiti, Polynésie française

[5]School of Biological Sciences, Victoria University of Wellington,Wellington, New Zealand

关键词: Domestication;    Temperature;    Transcriptomics;    Growth;    Sparidae;   

DOI  :  10.1534/g3.118.200647

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

Identifying genes and pathways involved in domestication is critical to understand how species change in response to human-induced selection pressures, such as increased temperatures. Given the profound influence of temperature on fish metabolism and organismal performance, a comparison of how temperature affects wild and domestic strains of snapper is an important question to address. We experimentally manipulated temperature conditions for F₁-hatchery and wild Australasian snapper ( Chrysophrys auratus ) for 18 days to mimic seasonal extremes and measured differences in growth, white muscle RNA transcription and hematological parameters. Over 2.2 Gb paired-end reads were assembled de novo for a total set of 33,017 transcripts (N50 = 2,804). We found pronounced growth and gene expression differences between wild and domesticated individuals related to global developmental and immune pathways. Temperature-modulated growth responses were linked to major pathways affecting metabolism, cell regulation and signaling. This study is the first step toward gaining an understanding of the changes occurring in the early stages of domestication, and the mechanisms underlying thermal adaptation and associated growth in poikilothermic vertebrates. Our study further provides the first transcriptome resources for studying biological questions in this non-model fish species.

【 授权许可】

CC BY|CC BY-NC