Genome-Wide Analysis of Multidrug and Toxic Compound Extrusion (MATE) Family in Gossypium raimondii and Gossypium arboreum and Its Expression Analysis Under Salt, Cadmium, and Drought Stress

G3: Genes, Genomes, Genetics

article

Pu Lu¹  Richard Odongo Magwanga¹  Xinlei Guo¹  Joy Nyangasi Kirungu¹  Hejun Lu¹  Xiaoyan Cai¹  Zhongli Zhou¹  Yangyang Wei³  Xingxing Wang¹  Zhenmei Zhang¹  Renhai Peng³  Kunbo Wang¹  Fang Liu¹ 

[1] Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton Biology/Institute of Cotton Research,Chinese Academy of Agricultural Science, Anyang 455000, Henan, China;School of Physical and Biological Sciences,Jaramogi Oginga Odinga University of Science and Technology, 40601 Bondo, Kenya;Biological and Food Engineering, Anyang Institute of Technology, Anyang 455000, Henan, China

关键词: MATE genes;    Gossypium arboreum;    Gossypium raimondii;    phylogenetic tree analysis;    GO annotation;    cadmium;    drought;    stress;    GenPred;    Shared Data Resources;    Genomic Selection;   

DOI  :  10.1534/g3.118.200232

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

The extrusion of toxins and substances at a cellular level is a vital life process in plants under abiotic stress. The multidrug and toxic compound extrusion ( MATE ) gene family plays a large role in the exportation of toxins and other substrates. We carried out a genome-wide analysis of MATE gene families in Gossypium raimondii and Gossypium arboreum and assessed their expression levels under salt, cadmium and drought stresses. We identified 70 and 68 MATE genes in G. raimondii and G. arboreum , respectively. The majority of the genes were predicted to be localized within the plasma membrane, with some distributed in other cell parts. Based on phylogenetic analysis, the genes were subdivided into three subfamilies, designated as M1, M2 and M3. Closely related members shared similar gene structures, and thus were highly conserved in nature and have mainly evolved through purifying selection. The genes were distributed in all chromosomes. Twenty-nine gene duplication events were detected, with segmental being the dominant type. GO annotation revealed a link to salt, drought and cadmium stresses. The genes exhibited differential expression, with GrMATE18 , GrMATE34 , GaMATE41 and GaMATE51 significantly upregulated under drought, salt and cadmium stress, and these could possibly be the candidate genes. Our results provide the first data on the genome-wide and functional characterization of MATE genes in diploid cotton, and are important for breeders of more stress-tolerant cotton genotypes.

【 授权许可】

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