CORL Expression and Function in Insulin Producing Neurons Reversibly Influences Adult Longevity in Drosophila

G3: Genes, Genomes, Genetics

article

Nancy L. Tran¹  Samuel L. Goldsmith¹  Agapi Dimitriadou²  Norma T. Takaesu¹  Christos Consoulas²  Stuart J. Newfeld¹ 

[1] School of Life Sciences, Arizona State University, Tempe AZ 85287-4501;Medical School, National and Kapodistrian University of Athens, Athens, Greece

关键词: Fussel/SKOR;    dILP2;    Drifter;    pars intercerebralis;    lifespan extension;   

DOI  :  10.1534/g3.118.200572

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

CORL proteins (known as SKOR in mice, Fussel in humans and fussel in Flybase) are a family of CNS specific proteins related to Sno/Ski oncogenes. Their developmental and adult roles are largely unknown. A Drosophila CORL (dCORL) reporter gene is expressed in all Drosophila insulin-like peptide 2 (dILP2) neurons of the pars intercerebralis (PI) of the larval and adult brain. The transcription factor Drifter is also expressed in the PI in a subset of dCORL and dILP2 expressing neurons and in several non-dILP2 neurons. dCORL mutant virgin adult brains are missing all dILP2 neurons that do not also express Drifter. This phenotype is also seen when expressing dCORL-RNAi in neurosecretory cells of the PI. dCORL mutant virgin adults of both sexes have a significantly shorter lifespan than their parental strain. This longevity defect is completely reversed by mating (lifespan increases over 50% for males and females). Analyses of dCORL mutant mated adult brains revealed a complete rescue of dILP2 neurons without Drifter. Taken together, the data suggest that dCORL participates in a neural network connecting the insulin signaling pathway, longevity and mating. The conserved sequence and CNS specificity of all CORL proteins imply that this network may be operating in mammals.

【 授权许可】

CC BY|CC BY-NC