Advances in Engineering the Fly Genome with the CRISPR-Cas System

Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation

article

Ethan Bier¹  Melissa M. Harrison²  Kate M. O’Connor-Giles³  Jill Wildonger⁴ 

[1] Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093-0349;Department of Biomolecular Chemistry,University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706;Laboratory of Genetics and Laboratory of Cell and Molecular Biology,University of Wisconsin-Madison, Wisconsin 53706;Biochemistry Department, University of Wisconsin-Madison, Wisconsin 53706

关键词: Drosophila;    CRISPR-Cas;    gene editing;    genome engineering;    gene drive;    FlyBook;   

DOI  :  10.1534/genetics.117.1113

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate relationships between genotype and phenotype, to delineate and visualize how the genome is organized, to illuminate and manipulate RNA, and to pioneer new gene drive technologies. Myriad transformative approaches have already originated from the CRISPR-Cas system, which will likely continue to spark the creation of tools with diverse applications. Here, we provide an overview of how CRISPR-Cas gene editing has revolutionized genetic analysis in Drosophila and highlight key areas for future advances.

【 授权许可】

CC BY