Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus

Revista Brasileira de Zootecnia (Print)

Huo, Sheng-dong¹  Abudureyimu, Ayimuguli¹  Liu, Jun-lin¹  Zhang, Guo-hua¹  Ma, Zhong-ren¹  Zhang, Tao-jie¹ 

[1] Northwest Minzu University, China

关键词: Bos grunniens;    gonadotropin-releasing hormone receptor;    immunofluorescence;    real-time PCR.;   

DOI  :  10.1590/rbz4720160360

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle and Leydig cells. Gonadotropin-releasing hormone receptor is located in the pineal gland, hypothalamus, pituitary, and gonad during estrus of yaks and is mainly expressed in the hypothalamus and ovaries during the estrus period.

【 授权许可】

Unknown