Diferenciación genética de tilapia roja y gris (Oreochromis niloticus) mediante microsatélites y marcadores SCAR como indicadores del sexo genético

Revista Peruana de Biologia

Sánchez-Tuesta, Linda¹  Prieto, Zulita¹  Arqueros, Monica¹ 

[1] Universidad Nacional de Trujillo, Perú

关键词: Tilapia;    Oreochromis;    microsatellites;    SCAR markers;    sex determination.;   

DOI  :  10.15381/rpb.v24i3.13900

学科分类：生物科学（综合）

来源: Universidad Nacional Mayor de San Marcos * Facultad de Ciencias Biologicas

【 摘 要 】

The aims of this work was to develop a standardized molecular protocols, to differentiate red and gray tilapia lineages using DNA microsatellites and to evaluate SCAR-5F-X/5R and SCAR-5F/5RY markers associated with the phenotypic sex of O. niloticus. The UNH106 microsatellite allowed differentiating genetically the lineages of red tilapia from gray. The markers UNH136, UNH115 and UNH995 presented monomorphic loci in both the red and gray tilapia in the population stock of the Centro Experimental de Genética of the Universidad Nacional de Trujillo. Fragment sizes for microsatellites and the reference gene β actin are described. The effectiveness of SCAR markers as informative in the determination of the genetic sex in XX and YY females and XY y YY males of red tilapia and XX females and XY males of gray tilapia was also confirmed.

【 授权许可】

CC BY-NC