Solid-state NMR study of discrete environments of bone mineral nanoparticles using phosphorus-31 relaxation

Journal of Applied Biomedicine

关键词: OAosteoarthritis;    OPSosteoporosis;    OPAosteopenia;    ssNMRsolid-state NMR;    CACcrystalline apatite core;    AHSLamorphous hydrated surface layer;    MASmagic angle spinning;    Min/matrixthe mineral-to-matrix ratio;    CO3/Pcarbonate-to-phosphate ratio;    XSTcrystallinity;    BMDbone mineral density;    PCAprincipal component analysis;    MSMEmulti-slice multi-echo;    BV/TVbone volume to total volume ratio;    BDBloch-decay (pulse-acquire NMR experiment);    FWHMfull width at half maximum;    DEXAdual-energy X-ray absorptiometry;    CrossLaps, β-CTXC-terminal telopeptide of type-I collagen;    Osteocalcinbone γ-carboxyglutamic acid-containing protein;    CVcoefficient of variation;    B/Aconcentration ratio of phosphorus in the AHSL and CPC locations;    MSAmeasure of sampling adequacy;    CPcross-polarization (polarization transfer in ssNMR);    HPDhigh-power decoupling;    Apatite;    Bone;    Nanomaterial;    Osteoarthritis;    Osteoporosis;    Nuclear magnetic resonance;   

DOI  :  10.1016/j.jab.2016.07.001

学科分类：医学（综合）

来源: Elsevier

【 摘 要 】

Bone mineral nanoparticles, residing between collagenous fibres, contain a crystalline apatite core (CAC) covered by an amorphous hydrated surface layer (AHSL). The main objectives of this work were to characterize the nanoparticles of human bone mineral and their discrete CAC and AHSL regions and relate this information to bone diseases. This pilot study involved 18 postmenopausal women suffering from osteoarthritis together with osteoporosis or osteopenia. Trabecular tissue specimens were acquired from the femoral intertrochanteric region during total hip arthroplasty. The specimens of whole bone were examined by 31P solid-state NMR (ssNMR) to measure the concentration ratio of phosphorus in the AHSL and CAC locations, together with their 31P linewidths and spin-lattice relaxation times. It was found that the AHSL region of the studied specimens contained 13–24% apatite phosphorus. The magnitude, hydration and structural order of AHSL and CAC were dependent on the progress of the pertinent diseases. Thus, the 31P ssNMR spectroscopy can be used ex vivo to selectively analyse the discrete nanoapatite environments in whole bone specimens and possibly assist in postoperative medical diagnostics.

【 授权许可】

Unknown