Two Lung Cancer Development-Related Genes, Forkhead Box M1 (FOXM1) and Apolipoprotein E (APOE), are overexpressed in Bronchial of Patients after Long-Term Exposure to Sulfur Mustard

Iranian Journal of Pharmaceutical Research

关键词: Apolipoprotein E;    Cancer;    Forkhead box M1;    Lung;    Oxidative stress;    Sulfur mustard;   

DOI  :  

学科分类：社会科学、人文和艺术（综合）

来源: Shaheed Beheshti Medical University * School of Pharmacy

【 摘 要 】

Sulfur mustard (SM) is a strong alkylating and mutagenic compound that targets humanairway system. We considered the expression of Forkhead box M1 (FOXM1) and apolipoproteinE (APOE) genes, which are responsible for cell proliferation, differentiation, tumorigenesis,and increased risk of lung cancer, in the lung bronchial tissue of patients exposed to SM.After performing pulmonary functional tests (PFTs), 11 human subjects (five controls and sixSM-exposed patients) were entered in this study. Total RNA were extracted from all biopsysamples and then cDNA was synthesised for each specimen using RT-PCR. Changes in geneexpression were measured using a RT2 Profiler ™PCR Array. PFTs have demonstrated moreobstructive and restrictive spirometric patterns among patients compared to the controls. Ahigher expression was recorded for both examined genes in bronchial of SM-exposed patients.Expression of FOXM1 and APOE genes in bronchial of the patients was significantly (p <0.001) overexpressed by 14.8316 and 3.9504-folds, respectively. Mustard lungs were associatedwith increased expression of FOXM1 and APOE genes, which suggests an increased risk oflung cancer among these patients. Since FOXM1 and APOE are considered as oxidative stressresponsive genes, we speculate that increased expression of these genes is more likely linked tooverproduction of reactive oxygen species (ROS) and oxidative stress (OS) in mustard lungs.Further studies are required at protein level among SM-exposed patients with lung cancer touse these genes as lung cancer biomarkers among these patients.

【 授权许可】

CC BY