Ameliorating effect of Curculigo orchoides on chromium(VI) induced oxidative stress via, modulation of cytokines, transcription factors and apoptotic genes

Journal of Applied Biomedicine

关键词: Potassium dichromate;    Oxidative stress;    C. orchoides;    Antioxidant enzymes;    Histopathology;   

DOI  :  10.1016/j.jab.2017.03.003

学科分类：医学（综合）

来源: Elsevier

【 摘 要 】

The effect of hydro-alcoholic extract of Curculigo orchoides on hexavalent chromium (Cr VI) induced toxicity in rats was investigated. Sub-acute toxicity studies were performed by OECD guidelines. K2Cr2O7 (30 mg/kg) was administered to all groups except control group for a period of 28 days by oral gavage. Control group received distilled water; treatment groups received C. orchoides (25, 50 and 100 mg/kg). Cr(VI) administration resulted in up-regulation of serum biochemical parameters such as alanine transaminase, aspartate transaminase, alkaline phosphatase, and tissue biochemical markers viz. lipid peroxidation and protein carbonyl content. C. orchioides (100 mg/kg) significantly decreased these enzyme levels. The activities of anti-oxidant enzymes like superoxide dismutase, catalase and glutathione S-transferase were significantly decreased by Cr(VI) administration (50.7%, 43.7% and 37.9%, respectively). Further, mRNA expression studies and histopathology studies confirmed Cr(VI) toxicity. In all cases, C. orchioides promoted significant restoration of enzyme levels in a dose dependent manner. These results suggest the ameliorating effect of C. orchoides on Cr(VI) induced oxidative stress is probably via, modulation of cytokines, transcription factors and apoptotic genes.

【 授权许可】

Unknown