Apigenin inhibits ethanol-induced oxidative stress and LPS-induced inflammatory cytokine production in cultured rat hepatocytes

Journal of Applied Biomedicine

关键词: ALDalcoholic liver disease;    BCNUcarmustine;    CYP2E1cytochrome P450 2E1;    DMSOdimethyl sulfoxide;    GRglutathione reductase;    GSHreduced glutathione;    GSH-PXglutathione peroxidase;    GSTglutathione S-transferase;    IL-6interleukin-6;    INHisoniazid;    LPSlipopolysaccharide;    MDAmalondialdehyde;    BNF-κBnuclear factor-κB;    ROSreactive oxygen species;    SODsuperoxide dismutase;    NTF-αtumor necrosis factor-α;    Apigenin;    CYP2E1;    Hepatocytes;    Inflammatory cytokines;    Oxidative stress;   

DOI  :  10.1016/j.jab.2017.11.006

学科分类：医学（综合）

来源: Elsevier

【 摘 要 】

Apigenin is a natural flavonoid compound that has antioxidative, anti-inflammatory, and hepatoprotective effects, but the underlying mechanisms are still unclear. In this study, the effects of apigenin on ethanol-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammatory cytokine production were examined in cultured rat hepatocytes. Following pretreatment of ethanol-stimulated hepatocytes with apigenin 6–24 mM for 2 h, the levels of cytochrome P450 2E1 (CYP2E1) protein expression and supernatant alanine aminotransferase and malondialdehyde were reduced (P < 0.05 or P < 0.01), while the activities of glutathione reductase and glutathione peroxidase were increased (P < 0.05 or P < 0.01). Likewise, the pretreatment of LPS-stimulated hepatocytes with the same concentrations of apigenin could decrease the levels of nuclear factor-κB protein expression and supernatant tumor necrosis factor-α and interleukin-6 (P < 0.05 or P < 0.01), and increase the level of IκB-α protein expression (P < 0.05 or P < 0.01). In all of these results, the concentration of 24 μM was the most effective. These findings demonstrate that apigenin may exert an inhibitory effect on ethanol-induced oxidative stress and LPS-induced inflammation in the cultured hepatocytes, and its mechanisms may be related to the reduction of CYP2E1 expression, increment of antioxidative ability, and regulation of inflammatory gene expression.

【 授权许可】

Unknown