Journal of Applied Biomedicine | |
Apigenin inhibits ethanol-induced oxidative stress and LPS-induced inflammatory cytokine production in cultured rat hepatocytes | |
关键词: ALDalcoholic liver disease; BCNUcarmustine; CYP2E1cytochrome P450 2E1; DMSOdimethyl sulfoxide; GRglutathione reductase; GSHreduced glutathione; GSH-PXglutathione peroxidase; GSTglutathione S-transferase; IL-6interleukin-6; INHisoniazid; LPSlipopolysaccharide; MDAmalondialdehyde; BNF-κBnuclear factor-κB; ROSreactive oxygen species; SODsuperoxide dismutase; NTF-αtumor necrosis factor-α; Apigenin; CYP2E1; Hepatocytes; Inflammatory cytokines; Oxidative stress; | |
DOI : 10.1016/j.jab.2017.11.006 | |
学科分类:医学(综合) | |
来源: Elsevier | |
【 摘 要 】
Apigenin is a natural flavonoid compound that has antioxidative, anti-inflammatory, and hepatoprotective effects, but the underlying mechanisms are still unclear. In this study, the effects of apigenin on ethanol-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammatory cytokine production were examined in cultured rat hepatocytes. Following pretreatment of ethanol-stimulated hepatocytes with apigenin 6â24 mM for 2 h, the levels of cytochrome P450 2E1 (CYP2E1) protein expression and supernatant alanine aminotransferase and malondialdehyde were reduced (P < 0.05 or P < 0.01), while the activities of glutathione reductase and glutathione peroxidase were increased (P < 0.05 or P < 0.01). Likewise, the pretreatment of LPS-stimulated hepatocytes with the same concentrations of apigenin could decrease the levels of nuclear factor-κB protein expression and supernatant tumor necrosis factor-α and interleukin-6 (P < 0.05 or P < 0.01), and increase the level of IκB-α protein expression (P < 0.05 or P < 0.01). In all of these results, the concentration of 24 μM was the most effective. These findings demonstrate that apigenin may exert an inhibitory effect on ethanol-induced oxidative stress and LPS-induced inflammation in the cultured hepatocytes, and its mechanisms may be related to the reduction of CYP2E1 expression, increment of antioxidative ability, and regulation of inflammatory gene expression.
【 授权许可】
Unknown
【 预 览 】
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