Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA

The British Journal of Nutrition

Yang-Yi Fan¹  Natividad R. Fuentes²  Tim Y. Hou³  Rola Barhoumi⁴  Xian C. Li⁵  Nicolaas E. P. Deutz⁶  Marielle P. K. J. Engelen⁷ 

[1] 1Program in Integrative Nutrition and Complex Diseases, Departments of Nutrition & Food Science, Texas A&M University, College Station, TX 77843, USA;2Faculty of Toxicology, Texas A&M University, College Station, TX 77843, USA;3Image Analysis Laboratory, Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA;4Immunobiology & Transplant Science Center, Houston Methodist, Houston, TX 77030, USA;5Center for Translational Research in Aging & Longevity, Department of Health and Kinesiology, Texas A&M University, College Station, TX 77843, USA;6Center for Translational Environmental Health Research, Texas A&M University, College Station, TX 77843, USA;7Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, TX 77843, USA

关键词: CD4+ T cells;    Generalised polarisation;    Giant plasma membrane vesicles;    Phase separation;   

DOI  :  10.1017/S0007114517003385

学科分类：卫生学

来源: Cambridge University Press

【 摘 要 】

Cell membrane fatty acids influence fundamental properties of the plasma membrane, including membrane fluidity, protein functionality, and lipid raft signalling. Evidence suggests that dietary n-3 PUFA may target the plasma membrane of immune cells by altering plasma membrane lipid dynamics, thereby regulating the attenuation of immune cell activation and suppression of inflammation. As lipid-based immunotherapy might be a promising new clinical strategy for the treatment of inflammatory disorders, we conducted in vitro and in vivo experiments to examine the effects of n-3 PUFA on CD4+ T cell membrane order, mitochondrial bioenergetics and lymphoproliferation. n-3 PUFA were incorporated into human primary CD4+ T cells phospholipids in vitro in a dose-dependent manner, resulting in a reduction in whole cell membrane order, oxidative phosphorylation and proliferation. At higher doses, n-3 PUFA induced unique phase separation in T cell-derived giant plasma membrane vesicles. Similarly, in a short-term human pilot study, supplementation of fish oil (4 g n-3 PUFA/d) for 6 weeks in healthy subjects significantly elevated EPA (20 : 5n-3) levels in CD4+ T cell membrane phospholipids, and reduced membrane lipid order. These results demonstrate that the dynamic reshaping of human CD4+ T cell plasma membrane organisation by n-3 PUFA may modulate down-stream clonal expansion.

【 授权许可】

CC BY