Habitual dietary fibre intake influences gut microbiota response to an inulin-type fructan prebiotic: a randomised, double-blind, placebo-controlled, cross-over, human intervention study

The British Journal of Nutrition

Genelle Healey¹  Rinki Murphy²  Christine Butts³  Louise Brough⁴ 

[1] 1School of Food and Nutrition, Massey Institute of Food Science and Technology, Massey University, Palmerston North 4442, New Zealand;2Food, Nutrition and Health, The New Zealand Institute for Plant & Food Research Limited, Palmerston North 4474, New Zealand;3Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1142, New Zealand;4Faculty of Life Sciences and Medicine, Diabetes and Nutritional Sciences Division, King’s College London, London SE1 9NH, UK

关键词: Habitual dietary fibres;    Responsiveness;    Gut microbiota;    Inulin-type fructan prebiotics;    16S rRNA gene sequencing;   

DOI  :  10.1017/S0007114517003440

学科分类：卫生学

来源: Cambridge University Press

【 摘 要 】

Dysbiotic gut microbiota have been implicated in human disease. Diet-based therapeutic strategies have been used to manipulate the gut microbiota towards a more favourable profile. However, it has been demonstrated that large inter-individual variability exists in gut microbiota response to a dietary intervention. The primary objective of this study was to investigate whether habitually low dietary fibre (LDF) v. high dietary fibre (HDF) intakes influence gut microbiota response to an inulin-type fructan prebiotic. In this randomised, double-blind, placebo-controlled, cross-over study, thirty-four healthy participants were classified as LDF or HDF consumers. Gut microbiota composition (16S rRNA bacterial gene sequencing) and SCFA concentrations were assessed following 3 weeks of daily prebiotic supplementation (Orafti® Synergy 1; 16 g/d) or placebo (Glucidex® 29 Premium; 16 g/d), as well as after 3 weeks of the alternative intervention, following a 3-week washout period. In the LDF group, the prebiotic intervention led to an increase in Bifidobacterium (P=0·001). In the HDF group, the prebiotic intervention led to an increase in Bifidobacterium (P<0·001) and Faecalibacterium (P=0·010) and decreases in Coprococcus (P=0·010), Dorea (P=0·043) and Ruminococcus (Lachnospiraceae family) (P=0·032). This study demonstrates that those with HDF intakes have a greater gut microbiota response and are therefore more likely to benefit from an inulin-type fructan prebiotic than those with LDF intakes. Future studies aiming to modulate the gut microbiota and improve host health, using an inulin-type fructan prebiotic, should take habitual dietary fibre intake into account.

【 授权许可】

CC BY