Chlamydia trachomatis vacuole maturation in infected macrophages

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: lysosome;    autophagy;    RAW cell;    elementary body;    reticulate body;    fluorescent chlamydia;    live imaging;    spinning disk confocal;   

DOI  :  10.1189/jlb.0711336

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Chlamydiatrachomatisisanobligateintracellularbacteriumresponsibleforoneofthemostcommonsexuallytransmitteddiseases.Inepithelialcells,C.trachomatisresidesinamodifiedmembrane‐boundvacuoleknownasaninclusion,whichisisolatedfromtheendocyticpathway.However,thematurationprocessofC.trachomatiswithinimmunecells,suchasmacrophages,hasnotbeenstudiedextensively.Here,wedemonstratedthatRAWmacrophageseffectivelysuppressedC.trachomatisgrowthandpreventedGolgistackdisruption,ahallmarkdefectinepithelialcellsafterC.trachomatisinfection.Next,wesystematicallyexaminedassociationbetweenC.trachomatisandvariousendocyticpathwaymarkers.Spinningdiskconfocaltime‐lapsestudiesrevealedsignificantandrapidassociationbetweenC.trachomatiswithRab7andLAMP1,markersoflateendosomesandlysosomes.Moreover,pretreatmentwithaninhibitoroflysosomeacidificationledtosignificantincreasesinC.trachomatisgrowthinmacrophages.Atlaterstagesofinfection,C.trachomatisassociatedwiththeautophagymarkerLC3.TEManalysisconfirmedthatasignificantportionofC.trachomatisresidedwithindouble‐membrane‐boundcompartments,characteristicofautophagosomes.Together,theseresultssuggestthatmacrophagescansuppressC.trachomatisgrowthbytargetingitrapidlytolysosomes;moreover,autophagyisactivatedatlaterstagesofinfectionandtargetssignificantnumbersoftheinvadingbacteria,whichmayenhancesubsequentchlamydialantigenpresentation...

【 授权许可】

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