Differential contribution of dendritic cell CD1d to NKT cell‐enhanced humoral immunity and CD8+ T cell activation

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: antibody;    α;    ‐;    galactosylceramide;    antigen presentation;    adaptive immunity;   

DOI  :  10.1189/jlb.1111559

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

CD1d‐restrictedtypeINKTcellsprovidehelpforspecificantibodyproduction.Bcells,whichhavecapturedandpresentedaT‐dependent,antigen‐derivedpeptideonMHCclassIIandCD1d‐bindingglycolipidα‐GConCD1d,respectively,activateThandNKTcellstoelicitBcellhelp.However,theroleoftheDCCD1dinhumoralimmunityremainsunknown.WethereforeconstructedmixedbonemarrowchimerascontainingCD1d‐expressing,DTR‐transgenicDCsandCD1d+orCD1d−nontransgenicDCs.FollowingDT‐mediatedDCablationandimmunization,weobservedthattheprimaryandsecondaryantibodyresponseswereequivalentinthepresenceofCD1d+andCD1d−DCs.Incontrast,atotalablationofDCsdelayedtheprimaryantibodyresponse.FurtherexperimentsrevealedthatdepletionofCD1d+DCsblockedinvivoexpansionofantigen‐specificcytotoxic(CD8+)Tlymphocytes.TheseresultsprovideacleardemonstrationthatalthoughCD1dexpressiononDCsisessentialforNKT‐enhancedCD8+Tcellexpansion,itisdispensableforspecificantibodyproduction...

【 授权许可】

CC BY