STAT1‐dependent IgG cell‐surface expression in a human B cell line derived from a STAT1‐deficient patient

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: adaptive immunity;    innate immunity;   

DOI  :  10.1189/jlb.1109714

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

STAT1isakeyeffectorofcytokinesinvolvedintheresistancetopathogens;itsidentifiedtranscriptionaltargetsmediatetheinnateimmuneresponseinvolvedinthedefenseagainstvirusesandbacteria.LittleisknownabouttheroleofSTAT1inadaptiveimmunity,includingitsimpactonBCRorsurfaceIgexpression.Analysisofthispointisdifficultinhumans,asSTAT1deficiencyisextremelyrare.SDpatientsdieearlyinchildhoodfromasevereimmunodeficiency.Herein,aSDBcelllineobtainedfromaSDpatientwascomparedwithaBcelllinefromaSTAT1‐proficientsubjectinsearchofdifferencesinsurfaceIgexpression.InthisSDBcellline,acompleteabsenceofsurfaceIgGwasnoted.ThemRNAencodingthesurfaceformofIgGwasdetectedonlyinSTAT1‐proficientBcells;themRNAsencodingthesecretedandthesurfaceformsweredetectedinSDandSTAT1‐proficientBcells.Re‐expressionofSTAT1inSDBcellsrestoredsurfaceIgGexpressionandafunctionalBCR.Conversely,shRNAsilencingofSTAT1inBcellsreducedconsiderablytheexpressionofthesurfaceIgG.AlthoughlimitedtooneBcellline,theseresultssuggestthatSTAT1mayplayanessentialroleinsurfaceIgGexpressioninhumanBcells.PossiblemechanismsinvolveregulationofmRNAsplicing,transcription,orboth.TheseobservationsextendtheroleofSTAT1furtherinadaptiveimmunity,includingtheregulationofBCRexpression...

【 授权许可】

CC BY