Tissue‐resident mesenchymal stem cells attract peripheral blood neutrophils and enhance their inflammatory activity in response to microbial challenge

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: endotoxin;    infection;    inflammation;   

DOI  :  10.1189/jlb.0410207

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

HumanMSCsmayrespondtoTLRligation,andrecentresearchhassuggestedthatmanytissuescontaintissue‐specificMSCs,possiblylocatedinperiendothelialandperivascularregions.Atpresent,thefunctionalconsequencesofthesefindingsareunclear.Wehypothesizedthattissue‐specificMSCscouldplayaninstructionalroleduringearlyphasesofbacterialchallenge.Toinvestigatethishypothesisfurther,wesetupacoculturesystemofglandularMSCsandperipheralbloodneutrophilssothatwecouldanalyzethecellularinteractionsofthesecellsinresponsetoLPSchallenge.WefoundthatstimulationwithbacterialendotoxininducedchemokinereceptorexpressionandmobilityofMSCs.ActivatedMSCssecretedlargeamountsofinflammatorycytokinesandrecruitedneutrophilsinanIL‐8‐andMIF‐dependentmanner.Recruitedandactivatedneutrophilsshowedaprolongedlifespan,anincreasedexpressionofinflammatorychemokines,andanenhancedresponsivenesstowardsubsequentchallengewithLPS.Ourfindingsdemonstrateacomplex,functionalinteractionbetweentissue‐residentMSCsandperipheralbloodneutrophilsuponbacterialchallengeandsuggestaroleforMSCsintheearlyphasesofpathogenchallenge,whenclassicalimmunecellshavenotbeenrecruitedyet...

【 授权许可】

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