Effects of alcohol withdrawal on monocyte subset defects in chronic alcohol users

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: TLR;    cytokines;    immune function;   

DOI  :  10.1189/jlb.5A0216-060RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Excessivealcoholconsumptionhasamodulatingeffectonimmunefunctionsthatmaycontributetodecreasedimmunityandhostdefense.Itisassociatedwithincreasedintestinalpermeabilitytoendotoxinsthatisnormalizedafter14dofabstinence.WhetherandhowbloodmonocytesubsetsareimpairedinpatientswithanAUDandwhattheirevolutionisafteralcoholwithdrawalarethepaper'sobjectives.Withtheuseofflowcytometry,bloodmonocytesubsetswerequantifiedinAUDsbefore(n=40)and2wkafter(n=33)alcoholwithdrawalandcomparedwithHCdonors(n=20).ExpressionofTLR2andTLR4onmonocytesubsetswasalsoquantified.CytokineresponseofmonocyteswasmonitoredfollowingPGNandLPSstimulation.TheCD14+CD16−subsetwasdecreased,whereastheCD14dimCD16+subsetwasexpanded(P<0.001)inAUDcomparedwithHC.ThefrequenciesofTLR2‐andTLR4‐expressingmonocyteswerereducedinAUDcomparedwithHC.AlthoughthebasalproductionofIL‐1,IL‐6,andTNFbymonocytesinAUDwascomparedwithHC,thePGN‐andLPS‐mediatedIL‐6andTNFproductionwasincreasedinAUD.FrequenciesofIL‐6‐expressingmonocyteswerehigherinAUDthanHC.AlcoholwithdrawalpartiallyrestoredthedistributionofmonocytesubsetsandthefrequencyofIL‐6‐producingmonocytesandincreasedthefrequencyofTNF‐producingcellsinresponsetoLPSandPGNstimulationtolevelscomparedwiththoseinHC.Ourfindingsindicatethatchronicalcoholusealtersthedistributionaswellasthephenotypicandfunctionalcharacteristicsofbloodmonocytesubsets,whicharepartiallyrestoredfollowing2wkofalcoholwithdrawal...

【 授权许可】

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