Kupffer cells express a unique combination of phenotypic and functional characteristics compared with splenic and peritoneal macrophages

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: IL‐;    10;    immunoregulation;    liver;    endocytosis;   

DOI  :  10.1189/jlb.1111566

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TheimmunostimulatoryroleofKupffercellsinvariousinflammatoryliverdiseasesisstillnotfullyunderstood.Inthisstudy,phenotypicandfunctionalaspectsofKupffercellsfromhealthyC57BL/6micewereanalyzedandcomparedwiththoseofsplenicandperitonealmacrophagestogenerateablueprintofthecellsundersteady‐stateconditions.Inthemouseliver,onlyonepopulationofKupffercellswasidentifiedasF4/80highCD11blowcells.WeobservedthatfreshyisolatedKupffercellsareendocyticandshowarelativelyhighbasalROScontent.Interestingly,despiteexpressionofTLRmRNAonKupffercells,ligationofTLR4,TLR7/8,andTLR9resultedinaweakinductionofIL‐10,loworundetectablelevelsofIL‐12p40andTNF,andup‐regulationofCD40onthesurface.Kupffercellsandsplenicmacrophagesshowfunctionalsimilarities,incomparisonwithperitonealmacrophages,asreflectedbycomparablelevelsofTLR4,TLR7/8,andTLR9mRNAandloworundetectablelevelsofTNFandIL‐12p40produceduponTLRligation.Theunique,functionalcharacteristicsofKupffercells,demonstratedinthisstudy,suggestthatKupffercellsundersteady‐stateconditionsarespecializedasphagocytestoclearanddegradeparticulatesandonlyplayalimitedimmunoregulatoryroleviathereleaseofsolublemediators...

【 授权许可】

CC BY