Staphylococcus aureus SaeR/S‐regulated factors reduce human neutrophil reactive oxygen species production

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: innate immunity;    bacteria;    host‐;    pathogen interactions;    two‐;    component systems;   

DOI  :  10.1189/jlb.4VMAB0316-100RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Neutrophilsarethefirstlineofdefenseafterapathogenhasbreachedtheepithelialbarriers,andunimpairedneutrophilfunctionsareessentialtoclearinfections.Staphylococcusaureusisaprevalenthumanpathogenthatisabletowithstandneutrophilkilling,yetthemechanismsusedbyS.aureustoinhibitneutrophilclearanceremainincompletelydefined.Theproductionofreactiveoxygenspecies(ROS)isavitalneutrophilantimicrobialmechanism.Herein,wetestthehypothesisthatS.aureususestheSaeR/Stwo‐componentgeneregulatorysystemtoproducevirulencefactorsthatreduceneutrophilROSproduction.WiththeuseofROSprobes,thetemporalandoverallproductionofneutrophilROSwasassessedduringexposuretotheclinicallyrelevantS.aureusUSA300(strainLAC)anditsisogenicmutantLACΔsaeR/S.OurresultsdemonstratedthatSaeR/S‐regulatedfactorsdonotinhibitneutrophilsuperoxide(O2−)production.However,subsequentneutrophilROSproductionwassignificantlyreducedduringexposuretoLACcomparedwithLACΔsaeR/S.Inaddition,neutrophilH2O2productionwasreducedsignificantlybySaeR/S‐regulatedfactorsbyamechanismindependentofcatalase.Consequently,thereductioninneutrophilH2O2resultedindecreasedproductionofthehighlyantimicrobialagenthypochlorousacid/hypochloriteanion(HOCl/−OCl).ThesefindingssuggestanewevasionstrategyusedbyS.aureustodiminishavitalneutrophilantimicrobialmechanism...

【 授权许可】

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