Characterization of lung infection–induced TCRγδ T cell phenotypes by CyTOF mass cytometry

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: pneumonia;    immune response;    IFN‐;    γ;    IL‐;    17;   

DOI  :  10.1189/jlb.4A0315-115RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TcellreceptorγδcellsareknowntobetheprimaryeffectorTcellsinvolvedintheresponsetobacterialinfections,yettheirphenotypiccharacteristicsarenotaswellestablishedasotherTcellsubsets.Inthisstudy,weusedcytometrybytime‐of‐flightmasscytometrytobettercharacterizethephenotypicresponseofTcellreceptorγδcellstoStreptococcuspneumoniaelunginfection.Micewereinfected,andcellsfromlungwashouts,spleen,andlymphnodeswerestainedtodetectcell‐surface,intracellular,andsignalingmarkers.WeobservedthatinfectioncausedasignificantincreaseinTcellreceptorγδcells,whichexpressedhighinterferon‐γandinterleukin‐17Alevels.ProfilingTcellreceptorγδcellsbycytometrybytime‐of‐flightrevealedthatactivatedγδTcellsuniquelycoexpressedcell‐surfaceGr‐1,clusterofdifferentiation14,andclusterofdifferentiation274(programmeddeath‐ligand1).FurtherclassificationofGr‐1expressionpatternsonTcellreceptorγδcellsdemonstratedthatGr‐1+Tcellreceptorγδcellsweretheprimarysourceofinterferon‐γ,whereasGr‐1−cellsmostlyexpressedinterleukin‐17A.Gr‐1+Tcellreceptorγδcellsalsoshowedhigherζ‐chain–associatedproteinkinase70,p38,and4eBP1signalinginresponsetoinfectionascomparedwithGr‐1−Tcellreceptorγδcells.Takentogether,Gr‐1expressionpatternsonγδTcellsinthelungprovidearobustmarkertodifferentiateinterferon‐γ–andinterleukin‐17A–producingsubsetsinvolvedintheearlyimmuneresponsetobacterialpneumonia...

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