Dendritic cell function at low physiological temperature

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: skin;    cell activation;    antigen presentation;   

DOI  :  10.1189/jlb.0310155

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Comparedwiththestablecoretemperature,theskintemperatureislowerandvariesdependingonambienttemperatureandconvectionconditions.ThefunctionofDCs,whichareplentifulintheskinatlowerphysiologicaltemperatures,hasnotbeenreported.WeshowthatDCperformedsomefunctionsnormallyat28°C,includingphagocytosisandmacropinocytosis.TLR‐4signalingviaMAPKpathwayswasdelayedat28°Cbutreachednormallevels,whichmayexplaintheobservedslowerkineticsofstimulatedmacropinocytosisandTNFproduction.TLR‐4‐inducedNOproductionwascompromisedseverelyat28°C.Collagendegradationandmigrationthroughmatrigel‐coatedtranswellinsertsweredecreased,butnoeffectonpodosomenumberorDCmigrationthroughnoncoatedtranswellfilterswasseen.LoweringthetemperaturedifferentiallyregulatedfunctionsassociatedwiththeroleofDCsinadaptiveimmunity.LPS‐inducedup‐regulationofCD86wasnormal;however,CD40up‐regulationwassuppressedafterTLR‐4stimulationat28°C.NonactivatedDCprocessedandpresentedantigenonMHCclassIIequallywellat28°Cand37°C.However,DCsthatwereloadedwithantigensandstimulatedwithTLRligandat28°CwerepooratactivatingTcellsat37°CcomparedwithDCsthatwereactivatedandloadedwithantigenat37°C...

【 授权许可】

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