| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Technical Advance: Autofluorescence as a tool for myeloid cell analysis | |
| 关键词: macrophage; monocyte; flow cytometry; spleen; | |
| DOI : 10.1189/jlb.0310184 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
CellularAFisusuallyconsideredahindrancetoflowcytometricanalysis.Here,weincorporateAFintoanalysisofcomplexmixturesofleukocytes.Usingamousemodel,weexaminedcellularAFatmultipleexcitationandemissionwavelengths,andpopulationswithdiscretepatternsweregatedandexaminedforsurfacemarkerexpression.Inthespleen,allmajormyeloidpopulationswereidentified.Inparticular,theapproachallowedsimultaneouscharacterizationofRPMandresidentmonocytes.WhenmonocytesandRPMwerecompared,RPMexhibitedaphenotypethatwasconsistentwithinvolvementinphysiologicalprocesses,includingexpressionofgenesinvolvedinlipidandironmetabolism.ThepresenceoflargeamountsofstoredferricironwithinRPMenabledpurificationofthesecellsusingamagnetic‐basedapproach.Whenadaptedforuseonleukocytesisolatedfromarangeofotherorgans,incorporationofAFintoanalysisallowedidentificationandisolationofbiologicallyimportantmyeloidpopulations,includingsubsetsthatwerenotreadilyidentifiablebyconventionalcytometricanalysis...
【 授权许可】
CC BY
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201904045507895ZK.pdf | 2431KB |  download |
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