Potent intestinal Th17 priming through peripheral lipopolysaccharide‐based immunization

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: T Cells;    vaccination;    superantigens;    cell differentiation;   

DOI  :  10.1189/jlb.0909631

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Lipopolysaccharide(LPS)isapotentnaturaladjuvant,commonlyusedtoamplifyTh1responses.Here,wereportthatsystemicimmunizationusingLPSgenerateslargenumbersofspecificTh17cellsinmurinesmallintestinallaminapropria.TheprimingoftheseTh17cellsrequiredIL‐23p19productionbybonemarrow‐derivedcells.Incontrast,IL‐23hadnoimpactonTh1differentiationoroverallnumbersofAg‐specificregulatoryTcells.ExperimentsusingT‐celladoptivetransfersrevealedapreviouslyunappreciatedmechanismforhowTh17responsesareamplifiedinvivo:stimulationthroughLPSexpandedprecommittedTh17cellsratherthancausingTh17differentiation.Second,LPSdroveTh17cellexpansionindependentlyofIL‐23,demonstratingthatthiscytokineisnotnecessaryforexpansionandpossiblyfunctionsatanearlierstageinTh17priming.OurdataprovideanimpetusforusingLPS‐basedperipheralvaccinationtoaugmentspecificT‐cell‐mediatedimmunityinthegutmucosa...

【 授权许可】

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