The fast detection of streptavidin based on the initial reaction rate of the binding-induced DNA strand-displacement reaction

Analytical methods

Ruoyun Lin¹  Feng Liu¹  Tian Li¹  Na Li¹ 

[1] Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University,Beijing,China

DOI  :  10.1039/C6AY02058E

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

Binding-induced DNA strand-displacement reactions diversify the applications of DNA strand-displacement reactions beyond nucleic acids and small molecules. To gain an in-depth understanding of the reaction induced by the biotin-streptavidin interaction, we studied the kinetics of the reaction and established a protein detection method based on the binding-induced strand-displacement reaction rate...

【 授权许可】

CC BY-NC