All‐trans retinoic acid and rapamycin synergize with transforming growth factor‐β1 to induce regulatory T cells but confer different migratory capacities

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: Treg migration;    chemokines;    adoptive cell therapy;    inflammatory bowel disease;    live‐;    animal imaging;   

DOI  :  10.1189/jlb.0312167

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Tregsplayimportantrolesinmaintainingimmunehomeostasis,andthus,therapiesbasedonTregarepromisingcandidatesforthetreatmentforavarietyofimmune‐mediateddisorders.Thesetherapies,however,facethesignificantchallengeofobtainingadequatenumbersofTregsfromperipheralbloodthatmaintainssuppressivefunctionfollowingextensiveexpansion.InducingTregsfromnon‐Tregsoffersaviablealternative.DifferentmethodstoinduceTregshavebeenproposedandinvolvemainlytreatingcellswithTGF‐β‐iTreg.However,useofTGF‐βaloneisnotsufficienttoinducestableTregs.ATRAorrapahasbeenshowntosynergizewithTGF‐βtoinducestableTregs.WhereasTGF‐βplusRA‐iTregshavebeenwell‐describedintheliterature,thephenotype,function,andmigratorycharacteristicsofTGF‐βplusrapa‐iTreghaveyettobeelucidated.Herein,wedescribethephenotypeandfunctionofmouserapa‐iTregandrevealthatthesecellsdifferintheirinvivohomingcapacitywhencomparedwithmouseRA‐iTregandmouseTGF‐β‐iTreg.Thisdifferenceinmigratoryactivitysignificantlyaffectsthetherapeuticcapacityofeachsubsetinamousemodelofcolitis.WealsodescribethecharacteristicsofiTreggeneratedinthepresenceofTGF‐β,RA,andrapa...

【 授权许可】

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