CD14hiHLA‐DRdim macrophages, with a resemblance to classical blood monocytes, dominate inflamed mucosa in Crohnˈs disease

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: myeloid cells;    inflammation;    cytokines;    matrix metalloproteinases;   

DOI  :  10.1189/jlb.0113021

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

IntestinalMϕplayanimportantroleinmaintainingguthomeostasis.However,littleisknownaboutthesecells,theirprecursors,andtheirroleinintestinalinflammation.Here,wecharacterizetheCD14+mononuclearcellpopulationsinintestinalmucosaandbloodinpatientswithCD.AmongtheLPCD14+Mϕ,weidentifiedthreedistinctHLA‐DR+‐expressingsubsets.Comparedwithuninflamed,inflamedmucosacontainedamarkedincreaseintheproportionoftheCD14hiHLA‐DRdimcellularsubset.ThissubsetresembledtheclassicalbloodmonocyteswithlowCD16,HLA‐DR,andCX3CR1expression.ClassicalmonocytesmigratedefficientlytowardCCL2andreleasedthehighestlevelsofMMP‐1andproinflammatorycytokineswhenstimulatedwithimmunecomplexesorLPS.OurfindingsstronglysuggestthatitistheclassicalandnottheintermediateornonclassicalmonocytesthataretheprecursorstothedominatingintestinalCD14hiHLA‐DRdimsubset.ThisenhancesourunderstandingofCDpathologyandmayprovidenewoptionsintreatment...

【 授权许可】

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