A sensitive HPLC assay for quantitative determination of polyamidoamine dendrimers after pre-column derivatization using FITC

Analytical methods

Jian Wang¹  Jina Yang¹  Rui Wang¹ 

[1] Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University,Fuzhou,China

DOI  :  10.1039/C3AY41896K

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

A pre-column derivatization method was developed to analyze polyamidoamine (PAMAM) dendrimers. The fluorescein isothiocyanate (FITC) derivation method improved the retention of PAMAM dendrimers on the reversed-phase column of high performance liquid chromatography (RP-HPLC). The derivation benefited the UV-Vis detection of PAMAM, although the fluorescence of FITC was quenched by PAMAM dendrimers. The primary amine-terminated PAMAM dendrimers, generation 3 (PAMAM-NH2 (G3)), were quantitatively determined by UV-Vis spectroscopy over the concentration range of 10–80 μg mL−1, with the calibration curve expressed as A = 131.6C (μg mL−1) − 295.6 (R2 = 0.9901) and the detection limit of 2.7 μg mL−1. The binding ratio of FITC to PAMAM-NH2 (G3) was determined as 8 : 1 by liquid chromatography-electrospray ionization-ion trap mass spectrometry (LC-ESI-IT-MS)...

【 授权许可】

CC BY-NC