Mannose receptor and macrophage galactose‐type lectin are involved in Bordetella pertussis mast cell interaction

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: C‐;    type lectin;    cytokine;    ICAM1;    ERK;    NF‐;    κ;    B;   

DOI  :  10.1189/jlb.0313130

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

MastcellsarecrucialinthedevelopmentofimmunityagainstBordetellapertussis,andthefunctionofTLRsinthisprocesshasbeeninvestigated.Here,theinteractionbetweenmastcellsandB.pertussiswithanemphasisontheroleofCLRsisexamined.Inthisstudy,twoCLRs,MGLandMR,weredetectedforthefirsttimeonthesurfaceofmastcells.TheinvolvementofMRandMGLinthestimulationofmastcellsbyheat‐inactivatedBPwasinvestigatedbytheuseofblockingantibodiesandspecificcarbohydrateligands.ThecellwallLOSofBPwasalsoisolatedtoexploreitsroleinthisinteraction.Mastcellsstimulatedwithheat‐inactivatedBPorBPLOSinducedTNF‐α,IL‐6,andIFN‐γsecretion,whichwassuppressedbyblockingMRorMGL.InhibitionofCLRssignalingduringBPstimulationaffectedtheabilityofmastcellstopromotecytokinesecretioninTcellsbuthadnoeffectonthecell‐surfaceexpressionofICAM1.BlockingMRorMGLsuppressedBP‐inducedNF‐κBexpressionbutnotERKphosphorylation.SykwasinvolvedintheCLR‐mediatedactivationofmastcellsbyBP.BacterialrecognitionbyimmunecellshasbeenpredominantlyattributedtoTLRs;inthisstudy,thenovelroleofCLRsintheBP–mastcellinteractionishighlighted...

【 授权许可】

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