Phagocyte dysfunction in polymyalgia rheumatica and other age‐related, chronic, inflammatory conditions

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: aging;    burst;    innate immunity;    interleukin 8;    phagocytosis;    rheumatoid arthritis;   

DOI  :  10.1189/jlb.0113034

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Thisstudywasconductedtoevaluatephagocytefunctioninpatientswithage‐relatedchronicinflammatoryconditions.Itincluded95patientswithPMR,17withGCA,40withEORA,and25age‐matchedHCs.SerumIL‐8wasdeterminedwithabeadarray.Thechemotacticcapacity,phagocyticability,andoxidativeburstactivityofcirculatingleukocytesweredeterminedwithflowcytometrykits.PatientswithactivechronicinflammatorydiseasesshowedasignificantincreaseincirculatinglevelsofIL‐8thatremainedelevatedinpatientswithPMRorEORA,despitetreatment.NocorrelationwasfoundbetweencirculatingIL‐8andthemigratotycapacityofneutrophils.NeutrophilsfrompatientswithactiveEORAwithoutstimulusandafterfMLPstimulishowedahighercapacitytomigratethanthoseoftheHCs(P=0.033).ThephagocyticactivityofgranulocytesinthepatientswithGCAwassignificantlyhigherthanintheHCsandthepatientswithPMRorEORA(P<0.05).ThepercentageandMFIofphagocytesthatproduceROIswhenstimulatedwithEscherichiacoliwassignificantlyreducedinneutrophilsandmonocytesfromthepatientswithage‐restrictedinflammatoryconditions.Weconcludedthattheeffectorfunctionsofphagocytes,determinedtobechemotaxis,phagocytosis,andoxidativeburst,arederegulatedinage‐restrictedinflammatorydisordersandmayhaveapathogenicrole...

【 授权许可】

CC BY