MicroRNA‐16 affects key functions of human endothelial progenitor cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: transcriptomic;    microarrays;    cell cycle;    cell differentiation;   

DOI  :  10.1189/jlb.1012511

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ThecapacityofEPCstorepairinjuredtissuesislimited.TheroleofmiRNAsinEPCsislargelyunknown.WetestedwhethermiRNAsmaybeusefultoenhancetheregenerativecapacityofEPCs.EarlyEPCswereisolatedfromhumanPBMCs,andlateEPCswereamplifiedfromenrichedhumanperipheralCD34+cells.ExpressionprofilesofmiRNAsandmRNAswereobtainedbymicroarrays.AmongthemiRNAsdifferentiallyexpressedbetweenearlyandlateEPCs,fivemembersofthemiR‐16family(miR‐15a/‐15b/‐16/‐103/‐107)wereoverexpressedinearlyEPCs.Web‐accessibledatabasespredicted375genetargetsforthesefivemiRNAs.Amongthese,tworegulatorsofcellcycleprogression(CCND1andCCNE1)andoneassociatedgene(CDK6)werelessexpressedinearlyEPCs.Administrationofanti‐miR‐16inearlyEPCsenhancedtheexpressionofthesethreegenes,andadministrationofpre‐miR‐16inlateEPCsdecreasedtheirexpression.InearlyEPCs,antagonismofmiR‐16allowedforcell‐cyclere‐entry,stimulateddifferentiation,enhancedIL‐8secretion,andpromotedtheformationofcapillary‐likestructuresbyHUVECs.Inconclusion,miR‐16regulateskeybiologicalpathwaysinEPCs.ThismayhaveimportantimplicationstoenhancethecapacityofEPCstorepairinjuredtissues...

【 授权许可】

CC BY