HIV infection of macrophages is enhanced in the presence of increased expression of CD163 induced by substance P

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: AIDS;    neurocognitive impairment;    monocytes;    hemoglobin;    haptoglobin;   

DOI  :  10.1189/jlb.4AB0813-434RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ActivationofNK1RbySPcontributestoincreasedHIV‐1infectioninmacrophages.ThescavengerreceptorCD163isexpressedoncellsofmonocyte‐macrophageorigin.OurmaingoalwastodetermineifthereisinterplayamongSP,CD163expression,andHIVinfectioninmacrophages.WeshowedthatSPtriggersintracellularcalciumelevationandincreasedCD163expressioninhumanmonocytesinatime‐andconcentration‐dependentmanner.TheroleofCD163onHIVinfectionwasexaminedbyRT‐PCRinsortedmonocytes(CD163lowandCD163high)andinmacrophageshavingCD163knockeddownusingsiRNA.WefoundthattheproductivityofHIVinfectionwashigherinCD163highcells.Additionally,inmacrophageswithCD163expressionknockeddown,wefoundasignificantdecreaseofHIVinfection.Furthermore,Hb‐Hpcomplexes,whichfunctionasanendogenousligandforCD163,decreasedHIVinfectioninmacrophagesinadose‐dependentmanner.Thus,wedemonstratethatSPinduceshigherlevelsofCD163inmonocytesandthathighexpressionofCD163isassociatedwithincreasesHIVinfectioninmacrophages.Thus,inadditiontobeingaprognosticmarkerofHIVinfection,theexpressionofCD163onmacrophagesmaybecriticalinHIVimmunopathogenesis...

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