Restricted HIV‐1 replication in placental macrophages is caused by inefficient viral transcription

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: primary cells;    monocytes;    macrophages;    viral mRNA;    RT‐;    PCR;    Western blots;   

DOI  :  10.1189/jlb.0809556

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

HIV‐infectedPMshowrestrictedreplicationascomparedwithMDM.WeaimedtodetermineatwhatpointintheviralreplicationcyclethisrestrictionoccursinPMascomparedwithMDM.WeperformedAlu‐LTRPCRforproviralDNAtodetectdifferencesinHIVintegration,real‐timeRT‐PCRtomeasureenvandgagmRNAlevels,andWesternblotanalysistodetectdifferencesinviralproteinexpression.PMandMDMwereinfectedwithHIV‐1BaL,andDNAwasextractedafter24handat6daysp.i.forreal‐timePCRstudies.At6and12daysp.i.,cellswerelysedforWesternblotanalyses.WefoundnodifferenceinviralintegrationbetweenPMandMDMbutsignificantlylowerlevelsofviralproteingp120inPMthaninMDM.Real‐timeRT‐PCRanalysesrevealed24‐foldlessenvmRNAandtenfoldlessgagmRNAinPM.TheseresultssuggestthatHIV‐1restrictioninPMoccursattheleveloftranscription.Thisstudyissignificant,asitadvancesourunderstandingofHIV‐1infectioninPManditscontributiontodecreasedinuteroverticaltransmission...

【 授权许可】

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