| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Control of the proliferation of activated CD4+ T cells by connexins | |
| 关键词: connexin 43; T cell receptor; hemichannels; cysteine uptake; cell cycle; lymphocytes; gap junction; | |
| DOI : 10.1189/jlb.0909613 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
AsexpressionofCxsincellsoftheimmunesystemincreasesuponcellularactivation,weinvestigatedwhetherCxsandespeciallyCxHcsplayamajorroleduringTcell‐mediatedresponses.Inparticular,westudiedtheexpressionofCx43HcfollowingCD4+Tcellstimulationusingflowcytometry,real‐timePCR,andWesternblotanalysis.WeshowedthatexpressionofCx43anditsphosphorylatedisoformsincreasedinresponsetotheengagementofCD3andCD28.Cx43HcswerefoundtobeinvolvedinsustainingproliferationofTcells,asassessedbycellcyclestaining,thymidineincorporationassays,andCFSEanalysisofcellsexposedtomimeticpeptideinhibitorsoftheplasmamembraneCxchannelsandantibodiesgeneratedtoanextracellularregionofCx.ThereductionofTcellproliferationmediatedbyCxchannelinhibitorssuppressedcysteineuptakebutnotcytokineproduction.Weconcludethatuponantigenrecognition,TcellsrequireCxHctosustaintheirclonalexpansion...
【 授权许可】
CC BY
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201904040670016ZK.pdf | 1386KB |  download |
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