Enantioseparations of three amino alcohols using a di-n-butyl-L-tartrate–boric acid complex as the mobile phase additive by reversed-phase high performance liquid chromatography

Analytical methods

Gengliang Yang¹  Xiufeng Liu¹  Juan Yang¹  Qienan Lu¹  Yanan Zou¹  Qi Liu¹ 

[1]Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University,Baoding,ChinaKey Laboratory of Medical Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University,Baoding,China

DOI  :  10.1039/C3AY42210K

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

Three amino alcohols, propranolol, clenbuterol and cycloclenbuterol, were enantioseparated on an achiral column by reversed-phase high performance liquid chromatography (RP-HPLC). A chiral chromatographic system was established based on C18-bonded silica gel as the support and a self-prepared di-n-butyl-L-tartrate–boric acid complex as the chiral mobile phase additive. In order to obtain better enantioseparations, the influences of di-n-butyl-L-tartrate and boric acid concentrations, the type, concentration and pH of the buffer, as well as methanol content were extensively investigated. It was found that the mobile phase composition played an important role in improving the enantioseparations and all of the enantiomers could be baseline resolved under the optimized experimental conditions. Three pairs of enantiomers, which could not be separated with only di-n-butyl-L-tartrate, obtained good chiral separations using the complex chiral selector. The primary driving forces responsible for the chiral recognition were assumed to be the ion-pair interaction between the enantiomers and the chiral selector; and the interactions between the ion-pairs above and the stationary phase also played important roles for the chiral separations in RP-HPLC...

【 授权许可】

CC BY-NC