60kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC

Cellular Physiology and Biochemistry

关键词: 60 KDa Lysophospholipase;    Yeast two hybrid;    Erk;    EnaC;    Sodium Transport;    Sgk1;   

DOI  :  10.1159/000322326

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of ENaC-mediated sodium transport and is involved in the transduction of growth-factor-dependent cell survival and proliferation. The identification of molecular partners for Sgk1 is crucial for the understanding of its mechanisms of action. We performed a yeast two-hybrid screening based on a human kidney cDNA library to identify molecular partners of Sgk1. As a result the screening revealed a specific interaction between Sgk1 and a 60 kDa Lysophospholipase (LysoLP). LysoLP is a poorly characterized enzyme that, based on sequence analysis, might possess lysophospholipase and asparaginase activities. We demonstrate that LysoLP has indeed a lysophospholipase activity and affects metabolic functions related to cell proliferation and regulation of membrane channels. Moreover we demonstrate in the Xenopus oocyte expression system that LysoLP downregulates basal and Sgk1-dependent ENaC activity. In conclusion LysoLP may represent a new player in the regulation of ENaC and Sgk1-dependent signaling.

【 授权许可】

CC BY-NC-ND