Electrochemical Deposition of Protein‐conjugated Graphene by Pulse Reverse Technique

Bulletin of the Korean Chemical Society

Dinakaran Thirumalai¹  Seung‐²  Cheol Chang³ 

[1] Graduate Department of Chemical Materials Pusan National University Busan 46241 Republic of Korea;Institute of Bio‐Physio Sensor Technology Pusan National University Busan 46241 Republic of Korea

关键词: Electrochemical deposition;    Graphene oxide;    Cytochrome c;    Pulse reverse technique;   

DOI  :  10.1002/bkcs.11309

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

A simple electrochemical deposition method for the immobilization of protein‐conjugated graphene onto an electrode has been developed. A unique one‐step electrochemical deposition of cytochrome c (Cyt c) conjugated with electrochemically reduced graphene oxide (ErGO) onto the surface of glassy carbon electrode (GCE) without any cross‐linkers and the direct electron transfer between Cyt c and the electrode was obtained by the Cyt c–ErGO‐modified GCE. The characteristics of the ErGO–Cyt c‐modified GCE were compared with those of an ErGO‐modified GCE without Cyt c. The direct electron transfer rate constant (ket) was found to be 6.2/s. This approach provides an avenue for the immobilization of various proteins and enzymes with ErGO and could be expanded for diverse biosensor applications.

【 授权许可】

Unknown