The Double Edge of Reactive Oxygen Species as Damaging and Signaling Molecules in HL60 Cell Culture

Cellular Physiology and Biochemistry

关键词: Adaptive response;    Gene expression;    HL60;    Hormesis;    Oxidative damage;   

DOI  :  10.1159/000276558

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Aims Our aim was to establish the conditions in which reactive oxygen species produce pathological or hormetic effects on HL60 cells. Methods HL60 cells were treated with either single bouts (1, 10 and 100 µM) or a sustained production (0.1, 1.0 and 10.0 nM/s) of H₂O₂. Results Exposure to 10 and 100 µM H₂O₂ activated catalase, glutathione peroxidase and glutathione reductase through post-transcriptional mechanisms and induced oxidative modification of proteins. When cells where exposed to sustained H₂O₂ production, a clear dose-response effect was detected in the activity of the antioxidant enzymes catalase, glutathione peroxidase and Mn-SOD, with higher concentrations of H₂O₂ inducing greater enzyme activities. Catalase, HO-1, UCP-3, iNOS and PGC-1α expressions were activated after sustained exposure to 1 and 10 nM H₂O₂/s. Although the antioxidant defenses were activated, oxidative damage appeared in DNA and proteins in cells treated with 1 and 10 nM/s. Conclusions HL60 cells respond to exposure to sustained levels of H₂O₂ in a dose-response manner to H₂O₂ concentration by activating the expression and activity of the antioxidant machinery, although the activation of the antioxidant defenses is not enough to avoid the appearance of oxidative damage. Of the two designs proposed, continuous exposure seems to be more appropriate to study the antioxidant response of HL60 cells to H₂O₂.

【 授权许可】

CC BY-NC-ND