First Record of a NPV Isolated from Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) in Turkey and Its Molecular Identification According to the Partial lef-8 Gene

Turkish Journal of Biology

UMUT TOPRAK¹ 

关键词: Baculovirus;    NPV;    Spodoptera littoralis;    lef-8;    PCR;    Nucleotide sequence analysis;   

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is an important pest in Turkey and causes extensive economic losses in many cultivated crops. The viruses within the family Baculoviridae are specific pathogens of insects. The nucleopolyhedroviruses (NPVs), 1 of the 2 genera of this family, have been isolated from many insect orders, primarily from the lepidopterans. In our study, a local NPV was isolated from the larvae of Spodoptera littoralis fed in the cotton fields in southern Turkey (Mersin). For the molecular identification of the virus, polyhedra (occlusion bodies [OBs]) were isolated from insects and total DNA extraction was performed using a commercial kit from OBs. One of the genes involved in late gene expression found in the SpliNPV genome, lef-8, was partially amplified by PCR. Nucleotide sequence analysis of this region was performed by MWG (Ebersberg, Germany). According to the nucleotide sequences of partial lef-8, searches carried out with the updated GenBanks showed that the isolate was a Spodoptera littoralis NPV-B variant. This NPV was the fifth SpliNPV-B variant isolated worldwide and was named SpliNPV-TR1 isolate as it is the first record in Turkey of a NPV isolated from Spodoptera littoralis.

【 授权许可】

Unknown