Metal‐doped Nanoparticles for Detection of Carbohydrate Antigen 15‐3 in Human Serum Using a Sandwich‐Type ICP‐MS Immunoassay

Bulletin of the Korean Chemical Society

Hana Jang¹  H. B. Lim² 

[1] Department of Chemistry Dankook University Gyeonggi‐do 16890 Korea

关键词: Metal‐;    doped magnetic nanoparticles;    ;    ICP‐;    MS ;    Metal‐;    doped silica nanoparticles;    Carbohydrate antigen 15‐;    3;    Immunoassay;   

DOI  :  10.1002/bkcs.10884

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

This work presents a method to determine carbohydrate antigen 15‐3 (CA15‐3) in serum using sandwich‐type inductively coupled plasma-mass spectrometry immunoassay. For this, Cd, Cs‐doped magnetic nanoparticles (MNPs) for target extraction and ratiometric measurement to enhance the calibration linearity were synthesized by co‐precipitation, of which the size was in the size range of 21–24 nm. The numbers of doped Cd and Cs atoms were in the range of 2.5 × 103 to 3.5 × 103 per MNP. The new synthetic method simplifies the synthetic procedure significantly with high flexibility for doping. Rare earth metal‐doped silica NPs (SNPs), i.e., Gd, Eu, or Ce, were also synthesized for detection through metal‐chelation and microemulsion method, with a final size range of 70–85 nm. For application, carbohydrate antigen 15‐3 (CA15‐3) was spiked into a human serum and determined by the sandwich‐type ICP‐MS immunoassay using the synthesized nanoparticles after blank subtraction. The obtained limit of detection (LoD) without any sample treatment was 2.70 × 10−4 U/mL, which was about 2.28 × 104 times better than that of enzyme‐linked immunosorbent assay (ELISA).

【 授权许可】

Unknown