Enhancing Lysozyme Loading in Powderized Liposomes by Controlling Encapsulation Processes

Bulletin of the Korean Chemical Society

Changhee Park¹  Yup Yoo¹  Jaehwi Lee¹  Eun Lee¹  Chul Hong¹  Hyeongmin Kim¹  Seung‐²  Kyung Bin Lee²  Young‐²  Seong‐²  Jeong Tae Kim²  Jieun Ro² 

[1] Ang University Seoul 06974 Korea;College of Pharmacy Chung‐

关键词: Lysozyme;    Liposome;    Encapsulation;    Reverse phase evaporation method;    Protein therapeutics;   

DOI  :  10.1002/bkcs.11164

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Liposomes have demonstrated great potentials as protein carriers in various pharmaceutical applications. However, low loading amount of proteins in liposomes have been a major challenge for maximizing the therapeutics potential of the proteins. We thus aimed to enhance the loading amount of a model protein, lysozyme, in liposomes by controlling key experimental variables of a reverse phase evaporation method. The loading amount of lysozyme in liposomes was evaluated with changing type of organic solvents used, weight ratio of lysozyme/phosphatidylcholine, and volume ratio of aqueous to organic phase along with particle characterization before and after freeze‐drying procedure. As a result, the loading amount of lysozyme in liposomes was considerably enhanced and the physical stability of the liposomes was maintained without any significant changes in the particle characteristics for 7 days. The findings of this study would be useful for highly efficient loading of protein therapeutics in liposomes, leading to improved therapeutic effects of the drugs.

【 授权许可】

Unknown