Effects of storage conditions on the stability of serum CD163, NGAL, HMGB1 and MIP2

International Journal of Clinical and Experimental Pathology

Jing Wang¹ 

关键词: CD163;    NGAL;    HMGB1;    MIP-2;    temperature;    storage time;    repeated freezing-thawing;   

DOI  :  

学科分类：生理学与病理学

来源: e-Century Publishing Corporation

【 摘 要 】

Background: Several cytokines have been involved in the diagnosis and prognosis for the pathogenesis and severity of chronic hepatitis B (CHB) such as cluster of differentiation 163 (CD163), neutrophil gelatinase-associated lipocalin (NGAL), high-mobility group box 1 (HMGB1) and macrophage inflammatory protein-2 (MIP-2). Nevertheless, the stability and reliability of these cytokines can be greatly influenced by handling and storage processes. Methods: In this study, potential utility of serum samples of a CHB cohort was evaluated to investigate several processes that might impact cytokine profiles such as temperature, storage time and number of freeze-thaw cycles. Blood samples collected from 100 patients with CHB were separated immediately and divided into two groups. In one group, samples (n=50) stored at -80°C were subject to 1-3 freeze-thaw cycles. In the other group, samples (n=50) were stored at 4°C and 25°C for 3 h, 9 h, 24 h, 48 h, 72 h, and 7 d time points, respectively. To assess the influence of different storage conditions on cytokines, the levels of CD163, NGAL, HMGB1 and MIP-2 were measured using enzyme-linked immunosorbent assays (ELISA) kits. Results: No significant differences of these four cytokines after 1-3 repeated freeze-thaw cycles. Significant differences of NAGL levels were seen between 9 h and 7 d (P<0.05), and also in HMGB1 at 25°C, while the other cytokines were relatively stable at the two storage temperatures over the various time points. Conclusion: This study indicated that these four cytokines remained stable within three freeze-thaw cycles and 7 d at 4°C. No perceptible effects on CD163 and MIP-2 levels were presented under the storage condition of 7 d at room temperature, whereas the degradation of NGAL and HMGB1 were notable.

【 授权许可】

CC BY-NC