Suppression of Inflammatory Cytokine Secretion by an NF-ĸB Inhibitor DHMEQ in Nasal Polyps Fibroblasts

Cellular Physiology and Biochemistry

关键词: DHMEQ;    Fluticasone propionate;    Glucocorticoid;    Nasal polyps;    NF-kappaB;    Rhinosinusitis;    Dehydroxymethylepoxyquinomicin;    In vitro culture;    Polyps fibroblasts;    Topical steroid;   

DOI  :  10.1159/000339042

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background NF-ĸB is an essential transcription factor strongly associated to inflammatory response in chronic rhinosinusitis with nasal polyps (CRSwNP). DHMEQ is a NF-ĸB inhibitor that has been previously described with a greatpotential indecreasing inflammation in diseases other than CRSwNP. The aim of study isto evaluate the ability of DHMEQ to reducethe inflammatory recruiters on CRSwNP and to compare its anti-inflammatory profile as a single-agent or in association with fluticasone propionate (FP). Methods nasal polyp fibroblasts were cultured in TNF-α enriched media. Cells were submitted to three different concentrations (1, 10 and 100nM) of either FP, DHMEQ or both. Inflammatory response was accessed by VCAM-1, ICAM-1 and RANTES expression (by RTQ-PCR) and protein levels by ELISA. Nuclear translocation of NF-ĸB was also evaluated. Results both FP and DHMEQ inhibited inflammatory recruiters’ production and NF-ĸB nuclear translocation. Interestingly, the anti-inflammatory effect from the association steroids plus DHMEQ was more intense than of each drug in separate. Conclusion DHMEQ seems efficient in modulating the inflammatory process in CRSwNP. The synergic anti-inflammatory effect of DHMEQ and steroids may be a promising strategy to be explored, particularly in the setting of steroid-resistant NP.

【 授权许可】

CC BY-NC-ND