Lipopolysaccharide-induced Cyclooxygenase-2 Expression in Mouse Transformed Clara Cells

Cellular Physiology and Biochemistry

关键词: Prostaglandins;    Chemotaxis;    COX-2;    Clara cells;    LPS;    Inflammation;   

DOI  :  10.1159/000337602

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background/Aims Exacerbation of innate immune responses can contribute to development of acute lung injury. Multiple cell populations, including the bronchiolar epithelium, coordinate these inflammatory responses. Clara cells, non-ciliated epithelial cells, are located in the distal airways in humans and conducting airways in mice. These cells actively participate in innate immune responses but their precise contributions remain poorly defined. Methods To test the hypothesis that E. coli lipopolysaccaride (LPS) treatment stimulates production of pro-inflammatory mediators in mouse transformed Clara cells (MTCC), MTCC were treated with E. coli lipopolysaccaride (LPS). Results LPS increased COX-2 expression and stimulated production of prostaglandins, including prostaglandin E₂ (PGE₂). Enhanced mitogen activated protein kinase (MAPK) activation, nuclear factor-ĸB (NFĸB) activation, and chemokine production were observed in MTCC in response to LPS treatment. Conclusions While the role for Clara cells in the regulation of host defense and the progression of acute lung injury needs further characterization, our data suggests the importance of this unique cell population in the pathogenesis of LPS-induced acute lung injury.

【 授权许可】

CC BY-NC-ND