Sphingosine but not Sphingosine-1-phosphate Stimulates Suicidal Erythrocyte Death

Cellular Physiology and Biochemistry

关键词: Sphingosine;    Sphingosine phosphate;    Oxidative stress;    Cell volume;    Annexin;    Eryptosis;    Calcium;    Phosphatidylserine;   

DOI  :  10.1159/000331750

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Sphingosine kinase 1 phosphorylates sphingosine, which is converted to ceramide by ceramide synthetase. Ceramide triggers eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine (PS) exposure at the erythrocyte surface. Erythrocytes lack sphingosine phosphate-degrading enzymes and thus store large quantities of sphingosine phosphate. The present study explored the influence of sphingosine and sphingosine phosphate on eryptosis. [Ca²⁺]_i, was estimated from Fluo3 fluorescence, cell volume from forward scatter and PS exposure from annexin V-binding in FACS analysis. Sphingosine (0.1 – 10 µM) but not sphingosine-1- phosphate (0.1 – 10 µM) increased [Ca²⁺]_i, decreased cell volume and increased PS-exposure. The observations disclose sphingosine, but not sphingosine-1-phosphate, as a strong inducer of eryptosis.

【 授权许可】

CC BY-NC-ND