Neuromedin S Increases L-type Ca2+ Channel Currents Through Giα-protein and Phospholipase C-dependent Novel Protein Kinase C Delta Pathway in Adult Rat Ventricular Myocytes

Cellular Physiology and Biochemistry

关键词: Neuromedin S (NMS);    L-type Ca2+ channels;    Ventricular myocytes;   

DOI  :  10.1159/000341443

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Neuromedin S (NMS), a peptide structurally related to NMU, has been identified in the mammalian heart tissues. However to date, any role of NMS in cardiomyocytes and the relevant mechanisms still remain unknown. In this study, we identified a novel functional role of NMS in modulating L-type Ca²⁺ channels in adult rat ventricular myocytes, in which NMU type 2 receptors (NMUR2), but not NMUR1, are endogenously expressed. We found that NMS at 0.1 µM reversibly increased I_Ba by ∼29.7%. Intracellular infusion of GDP-β-S or a selective antibody raised against the G_i-protein blocked the stimulatory effects of NMS. The classical and novel protein kinase C (nPKC) antagonist calphostin C or chelerythrine chloride, as well as the phospholipase C (PLC) inhibitor U73122, abolished NMS responses, whereas a classical PKC antagonist Gö6976 or a PKA antagonist PKI 5-24 had no such effects. Pretreatment of cells with PKC-δ specific inhibitor rottlerin or intracellular application of a PKC-δ-derived inhibitory peptide, δV1-1, abolished NMS responses, while an inactive control peptide had no effects. In summary, NMS acting through NMUR2 increases I_Ba via a G_iα-protein-dependent PKC-δ pathway in rat ventricular myocytes.

【 授权许可】

CC BY-NC-ND