An immunohistological study of the presence of inflammatory cells in malignant brain tumors

Current Health Sciences Journal

DIANA STANCAreplacement¹ 

关键词: astrocytoma;    glioblastoma;    immunohistochemistry;    T-lymphocytes;    macrophages;   

DOI  :  10.12865/CHSJ.38.03.05

学科分类：医学（综合）

来源: Universitatea de Medicina si Farmacie din Craiova / University of Medicine and Pharmacy

【 摘 要 】

The purpose of this study is to appreciate if there is an immune response in malignant brain tumors. Material and methods Eleven astrocytomas of varying histological features were immunohistologically studied, using a panel of monoclonal antibody against CD20cy to highlight T-lymphocytes, CD3 for B-lymphocytes and CD68 as marker of the macrophages. Ki67 antibody was used as marker of proliferation. We evaluated the presence of these markers in peritumoral stroma and intratumoral tissue. Results In our study, none of the astrocytic tumors studied showed B-lymphocytes. The presence of T lymphocytes was evidenced in all 11 selected cases. While low-grade astrocytomas showed positive CD3 immunostaining only in tumoral cells, anaplastic astrocytomas and glioblastomas revealed positive immunostaining also in perivascular space. As it was expected, mean values for proliferative marker Ki-67 revealed that there was a significant difference between the indices of low- (grade II) and high-grad.

【 授权许可】

Unknown