Silencing of ADIPOQ Efficiently Suppresses Preadipocyte Differentiation in Porcine

Cellular Physiology and Biochemistry

关键词: Pig;    ADIPOQ;    Preadipocytes;    Adipocyte differentiation;    RNAi;   

DOI  :  10.1159/000343381

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Aims Our study aims to characterize the functions of the ADIPOQ gene in the process of fat deposition of pigs, thereby providing a basis for the use of this gene as a molecular marker for pork quality. Methods We used healthy Junmu1 piglets less than 7 days of age to establish an in vitro culture system for porcine preadipocytes. Chemically synthesized short hairpin RNAs (shRNA) were transfected into porcine preadipocytes to silence the expression of the ADIPOQ gene. We monitored preadipocyte differentiation and determined the levels of the adipocyte differentiation transcription factors lipoprotein lipase (LPL), peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid binding protein (AP2) mRNAs to investigate the effects of ADIPOQ on the differentiation of porcine preadipocytes. Results After transfection, the mRNA and protein levels of the ADIPOQ gene were significantly decreased (P < 0.01), the number of lipid droplets in the adipocytes was significantly reduced, the OD values reflecting the fat content were significantly decreased (P < 0.01), and the levels of LPL, PPARγ and AP2 were significantly reduced (P < 0.01). Conclusions These results suggest that interference with ADIPOQ gene expression can inhibit the differentiation of porcine preadipocytes.

【 授权许可】

CC BY-NC-ND