Phytase production from Lactobacillus brevis

Turkish Journal of Biology

MELİS SÜMENGEN¹ 

关键词: Lactobacillus brevis;    phytase;    phytic acid;    zymogram;   

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

In this study, the phytase from Lactobacillus brevis strains isolated from fermented food products was produced and characterized. Phytase production of this strain was determined by production of clear zones around the colonies on the sodium phytate containing medium, after 48 h of incubation at 30 °C. The molecular weight of the phytase from Lactobacillus brevis was estimated as 73 kDa and 34 kDa on SDS-PAGE. The optimum activity of Lactobacillus brevis extracellular and intracellular enzymes emerged at 120 °C, pH 3.4 and at 110 °C, pH 3.4, respectively. The extracellular enzyme from Lactobacillus brevis was 91% stable for 5 min at 90 °C. Lactobacillus brevis intracellular enzyme activity was significantly stimulated with HgCl2 (157%), MnCl2 (140%), ZnCl2 (121%), MgCl2 (133%), CuCl2 (131%), and FeCl2 (123%).

【 授权许可】

Unknown