Modeling and Virtual Screening of Antisense Peptides Targeting the Divergent Region of Tumor‐Associated MT1‐MMP Protein

Bulletin of the Korean Chemical Society

Lili Xiong¹  Canquan Mao¹  Yue Kang¹  Tingting Huang¹  Zhiyun Guo¹  Jian Cui¹  Zongying Liu¹  Bowen Tan¹  Yinxuan Peng¹  Xiaomin Liu¹  Li Zeng¹  Yijie Zhou¹  Zhilei Song¹  Fang Wu¹ 

[1] Laboratory of Molecular Evolution and Applied Biology School of Life Science and Engineering, Southwest Jiaotong University Chengdu 610031 China

关键词: ;    MT1‐;    MMP ;    Antisense peptide;    Virtual screening;    Docking;    Modeling;   

DOI  :  10.1002/bkcs.10421

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Membrane type‐1 matrix metalloproteinase (MT1‐MMP; also known as MMP14) is a key enzyme involved in tumor invasion and metastasis, and is a potential target for drug discovery for cancer therapy. However, till now there is no MT1‐MMP‐ or MMP‐based anticancer drugs in the market mainly because of the high conservation of the MMP family and also because there is no elucidated crystal structure for the mature MT1‐MMP. The modeling of the three‐dimensional structure of mature MT1‐MMP and the finding of MT1‐MMP targeted peptides by virtual screening are highly desired. In this study, the three‐dimensional structure of mature MT1‐MMP is constructed by homology and de novo modeling and later rationalized and optimized by molecular dynamics simulations. An antisense peptide library was constructed against the divergent sense peptide DEGTEEET in the specific region of MT1‐MMP, which was found by multiple alignment of the whole MMP family. The antisense peptide library was virtually screened against the constructed three‐dimensional model of MT1‐MMP. The top 20 novel peptides were further studied, which were found well docked with MT1‐MMP at the region of DEGTEEET, again confirming their specific binding to MT1‐MMP. Preliminary study of one of the top‐ranked peptide SFLLSPFV showed that it could inhibit the viabilities of MG63 and MDA‐MB‐231 tumor cells. We thus not only successfully modeled the three‐dimensional structure of mature MT1‐MMP but also provided a new way for the finding of peptide candidates targeting MT1‐MMP based on antisense peptide library.

【 授权许可】

Unknown