Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

PLoS One

Pu Chu¹  Yongjun He²  Rongzhan Guan²  Haidong Jiang²  Mingquan Shao²  Huan Jia² 

[1] Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China;State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing, China

关键词: Brassica;    Plant resistance to abiotic stress;    Leaves;    Chloroplasts;    Seedlings;    Photosynthesis;    Salinity;    Biosynthesis;   

DOI  :  10.1371/journal.pone.0144808

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Salt stress limits plant growth and crop productivity and is an increasing threat to agriculture worldwide. In this study, proteomic and physiological responses of Brassica napus leaves under salt stress were investigated. Seedlings under salt treatment showed growth inhibition and photosynthesis reduction. A comparative proteomic analysis of seedling leaves exposed to 200 mM NaCl for 24 h, 48 h and 72 h was conducted. Forty-four protein spots were differentially accumulated upon NaCl treatment and 42 of them were identified, including several novel salt-responsive proteins. To determine the functional roles of these proteins in salt adaptation, their dynamic changes in abundance were analyzed. The results suggested that the up-accumulated proteins, which were associated with protein metabolism, damage repair and defense response, might contribute to the alleviation of the deleterious effect of salt stress on chlorophyll biosynthesis, photosynthesis, energy synthesis and respiration in Brassica napus leaves. This study will lead to a better understanding of the molecular basis of salt stress adaptation in Brassica napus and provides a basis for genetic engineering of plants with improved salt tolerance in the future.

【 授权许可】

CC BY